Human Rheumatoid Factors with Restrictive Specificity for Rabbit Immunoglobulin G: Auto- and Multi-reactivity, Diverse VM Gene Segment Usage and Preferential Usage of VxIIlb
نویسندگان
چکیده
From the Departments of *Medicine, *Psychiatric Medicine, and SMicrobiolagy, and the IIUniversity of Virginia Cancer Center, University of Virginia School of Medicine, Charlottesville, Virginia 22908; the '{Human Immunology & Cancer Programs, Department of Medicine, University of Tennessee Medical Center~Graduate School of Medicine, Knoxville, Tennessee 37920; and the **Departments of Medicine and Microbiology, University of Alabama at Birmingham, and the Birmingham Veterans Administration Hospital, Birmingham, Alabama 35294
منابع مشابه
Human rheumatoid factors with restrictive specificity for rabbit immunoglobulin G: auto- and multi-reactivity, diverse VH gene segment usage and preferential usage of V lambda IIIb
To determine the molecular and functional properties of human rheumatoid factors (RF), we established stable hybridomas and Epstein-Barr virus-transformed B cell lines from the synovial fluid or peripheral blood of three patients with rheumatoid arthritis and one patient with systemic lupus erythematosus. 17 cell lines were obtained that produced high-titer immunoglobulin M (IgM) RF that reacte...
متن کاملIdentification of Synonymous Codon Usage Bias in the Pseudorabies Virus UL31 Gene
Background: Little knowledge of synonymous codon usage pattern of pseudorabies virus (PRV) genome, especially the UL31 gene in the process for its evolution is available. Objectives: In the present study, the codon usage bias between PRV UL31 sequence and the UL31-like sequences was identified. Materials and Methods: We used a comprehensive analysi...
متن کاملP-128: Optimization of Human LH Gene Expression by Codon Usage Adaptation in CHO Cell Line
a:4:{s:10:"Background";s:897:"Human luteinizing hormone (hLH) belongs to glycoprotein hormones which is composed of two non-covalently linked subunit, α and β. The α-subunit is similar in all glycoprotein hormones, whereas the β-subunit is conferring the hormonal specificity. This hormone has important roles in the growth and maturity of sexual organs and secondary sexual characteristics and st...
متن کاملMatching amino acid and nucleotide sequences of mouse rheumatoid factor CDRH3-FRH4 segments to other mouse antibodies with known specificities
Contact: [email protected] Rheumatoid factors are auto-antibodies which bind the Fc portion of autologous immunoglobulin G molecules. Since the etiology of the production of such autoantibodies is unclear, we try to match the amino acid and nucleotide sequences of their heavy chain third complementarity determining (CDRH3) and fourth framework (FRH4) regions to other antibodies with known specificit...
متن کاملRheumatoid factors from the peripheral blood of two patients with rheumatoid arthritis are genetically heterogeneous and somatically mutated.
We report the DNA sequences of the heavy and light chain immunoglobulin genes of 11 monoclonal rheumatoid factor (RF)-secreting lines derived from the peripheral blood of two patients with rheumatoid arthritis (RA). It is evident from immunogenetic analysis of these lines that RA-associated RF activity can arise from a wide variety of heavy and light chain genes and gene combinations. Although ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2003